培養大腸桿菌除了LB培養基，還有什麼其他的培養基嗎？

1樓：

Commonly used bacterial E.coli growth media

常見的大腸桿菌培養基

LB (Luria Bertani) Miller broth (1%NaCl)LB Miller肉湯培養基: 1% peptone, 0.5% yeast extract, and 1% NaCl

LB (Luria Bertani) Lennox Broth (0.5% NaCl)Lennox肉湯培養基: 1% peptone, 0.

5% yeast extract, and 0.5% NaCl

SOB medium (Super Optimal Broth): 2% peptone, 0.5% Yeast extract, 10mM NaCl, 2.

5mM KCl, 10mM MgCl2 , 10mM MgSO4

SOC medium (Super Optimal broth with Catabolic repressor): SOB + 20mM glucose2x YT broth (2x Yeast extract and Tryptone): 1.

6% peptone, 1% yeast extra6ct, and 0.5% NaCl

TB (Terrific Broth) medium: 1.2% peptone, 2.

4% yeast extract, 72 mM K2HPO4, 17 mM KH2PO4 and 0.4% glycerol

SB (Super Broth) medium: 3.2% peptone, 2% yeast extract, and 0.5% NaCl

2樓：雨花

大腸桿菌在哪都能長得很快，只要有碳源、氮源和一點鹽，生命力十分強悍。培養基僅僅是要求成分穩定能滅菌、透明易觀察。最早是用的肉湯，效能不錯，但製取實在太麻煩，含油脂還易氧化哈喇，所以就簡化成了人工配製的LB。

LB如此廣泛使用還是因為它配製簡單，成本低，實際上任何含氨基酸含碳源的東西都可以，幾乎所有食物製品都能養大腸桿菌。